Fig. 6From: Rejuvenated endothelial progenitor cells through overexpression of cellular prion protein effectively salvaged the critical limb ischemia in rats with preexisting chronic kidney diseaseIschemic to normal blood flow (INBF) ratio measured by laser Doppler scan at days 0, 1, 7 and 14 after left CLI induction. A–E Illustrating the laser Doppler finding of ratio of left hind limb (ischemia) to right hind limb (normal) blood flow (i.e., INBF) at day 0 prior to critical limb ischemia (CLI) procedure among the five groups. F Analytical result of ratio of INBF, p > 0.5. G–K Illustrating the laser Doppler finding of INBF at day 1 after CLI procedure among the five groups. L Analytical result of ratio of INBF, * versus †, p < 0.0001. M–Q Illustrating the laser Doppler finding of ratio of INBF at day 7 after CLI procedure among the five groups. R Analytical result of ratio of INBF, * versus other groups with different symbols (†, ‡, §, ¶), p < 0.0001. S–W Illustrating the laser Doppler finding of ratio of INBF at day 14 after CLI procedure among the FIVE groups. X Analytical result of ratio of INBF, * versus other groups with different symbols (†, ‡, §, ¶), p < 0.0001. Note that each rat was placed in a supine position and the tail in upward direction. Thus, the left side of animal indicated the left critical limb and the attenuated Doppler color, i.e., implicated a reduction in blood flow in left CLI area, was clearly observed. All statistical analyses were performed by one-way ANOVA, followed by Bonferroni multiple comparison post hoc test (n = 8 for each group). Symbols (*, †, ‡, §, ¶) indicate significance (at 0.05 level). L = left limb; R = limb; right SC = sham-operated control; CKD = chronic kidney disease; CLI = critical limb ischemia; EPCs = endothelial progenitor cells; PrPcOE-EPCs = overexpression of cellular prion protein in EPCs; and siPrnp-EPCs = knockdown of cellular prion protein in EPCsBack to article page