Fig. 2

Hypoxia-Induced Imbalance Expression of Transcriptome Factors in cMSCs. A Transcriptome gene expression analysis of ischemic cMSCs and sham cMSCs. Heatmap of shared upregulated or downregulated genes with logarithmic fold change. Downregulated genes are represented in blue, and upregulated genes are represented in red. B Dotplot of top 10 enriched GO terms. C List of the most significantly upregulated (left) and downregulated (right) transcripts upon ischemia assayed by RNA-seq. D List of the top TFs with enriched predicted binding sites among genes altered upon ischemia. Among these TFs, c-Myc and Oct4 had altered expression after induction of myocardial ischemia. E and F The mRNA and protein expression levels of these transcriptome factors were verified in both cMSCs using qRT-PCR E and immunoblotting F. All data are the means ± SEM. p < 0.05: * vs. sham (n = 10 per group), independent samples t test was used. (G and H) Different expression of inflammatory or angiogenic factors in cMSCs from the ischemic or sham hearts by immunofluorescence staining. Positive staining for IL6 (green) and TGF-β1 (red) indicates higher inflammation in ischemic cMSCs compared with sham cMSCs G. Lower expression of Oct4 (green) and higher expression of c-Myc (green) in ischemic cMSCs compared with sham cMSCs indicates ischemia inducing imbalance expression of transcriptome factors H. DAPI is used to counterstain the nucleus (blue). Representative images of at least 3 different cells are shown. cMSCs indicate cardiac mesenchymal stromal cells. DAPI, 4’, 6-diamidino-2-phenylindole