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Fig. 3 | Stem Cell Research & Therapy

Fig. 3

From: Oct4 cooperates with c-Myc to improve mesenchymal-to-endothelial transition and myocardial repair of cardiac-resident mesenchymal stem cells

Fig. 3Fig. 3

c-Myc/Oct4 induces divergent cellular angiogenesis, inflammation, and fibrosis. A Venn diagram showing the common pathways between upregulated cMSCsoec−Myc vs cMSCsCON and upregulated in cMSCsoeOct4 vs cMSCsCON. B Venn diagram showing the common pathways between upregulated cMSCsoec−Myc vs cMSCsCON and downregulated in cMSCsoeOct4 vs cMSCsCON. C Scatter plot analysis of hallmark genes in cMSCsoeOct4 and cMSCsoec−Myc. Green, black, and red indicate downregulated genes, relatively stable with less than two fold change, and upregulated genes by more than two folds in cMSCsoeOct4 compared to cMSCsoec−Myc, respectively. D Bar graph showing the top 10 common pathways from A and all common pathways in B differentially regulated pathways between cMSCsoec−Myc vs cMSCsCON and cMSCsoeOct4 vs cMSCsCON analyses. E mRNA levels of the indicated factors in cMSCs transfected with control vehicle, oec-Myc, or oeOct4. Values are relative to GAPDH. All data are the means ± SEM; statistical significance was evaluated using the unpaired two-tailed Student’s t test with Welch’s correction. Comparison of each genotype with its own control is indicated as follows: **P < 0.001, ***P < 0.001. Comparison of oec-Myc is indicated in the same manner but using the symbol “#”; ##P < 0.001, ###P < 0.001. F These cMSCs were examined via immunofluorescence for the expression of vascular endothelial marker vWF (green), inflammatory cell markers, IL-1α (red) and TNFα (green), and fibroblast markers, MMP2 (red) and MMP9 (green). Also shown is DAPI staining (nuclei; blue).

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