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Fig. 6 | Stem Cell Research & Therapy

Fig. 6

From: Oct4 cooperates with c-Myc to improve mesenchymal-to-endothelial transition and myocardial repair of cardiac-resident mesenchymal stem cells

Fig. 6

Overexpression of Oct4 and c-Myc in implanted cMSCs improves their myocardial repair in recipient rats 30 days after MI. A Kaplan–Meier survival rates. B–E Echocardiography of LVFS B, LVEDD C, LVEDV D, and LVAWd E before MI (pre-MI), 1d after MI (1d post-MI), and 30d after MI (30d post-MI). Transplantation of cMSCs with co-transfection c-Myc and Oct4 had higher survival rates and significant improvement in cardiac function and structural remodeling. However, this was canceled by inhibition of c-Myc or Oct4, which was not rescued by combining with Oct4 or c-Myc transfection. (F) Quantitation of infarct size as determined by TTC staining at 30d post-MI. All graphical data are the means ± SEM. Welch ANOVA analyses were performed. p < 0.05: *vs PBS injection, #vs. cMSCs therapy alone, Δvs. Transplantation of oec-Myc transfected cMSCs, vs. Transplantation of cMSCs transfected with oec-Myc plus oeOct4, §vs. Transplantation of cMSCs transfected with oec-Myc plus siOct4, vs. Transplantation of cMSCs transfected with sic-Myc, vs. Transplantation of cMSCs transfected with sic-Myc plus oeOct4, $vs. pre-MI, &vs. 1d post-MI. (BE): Pre-MI, n = 20 per group. At 1d post-MI, PBS injection, n = 19; cell therapy: vehicle-treated cMSCs, n = 20; oec-Myc-treated cMSCs, n = 20; oec-Myc plus oeOct4-treated PBMSCs, n = 20; oec-Myc plus siOct4-treated PBMSCs, n = 20; cMSCs treated with sic-Myc alone, n = 20; cMSCs treated with sic-Myc plus oeOct4, n = 19, cMSCs treated with sic-Myc plus siOct4, n = 20. At 30 d post-MI, PBS injection, n = 10; cell therapy: vehicle-treated cMSCs, n = 13; oec-Myc-treated cMSCs, n = 14; oec-Myc plus oeOct4-treated PBMSCs, n = 19; oec-Myc plus siOct4-treated PBMSCs, n = 13; cMSCs treated with sic-Myc alone, n = 13; cMSCs treated with sic-Myc plus oeOct4, n = 14, cMSCs treated with sic-Myc plus siOct4, n = 11. F n = 5, each group

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