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Fig. 8 | Stem Cell Research & Therapy

Fig. 8

From: Oct4 cooperates with c-Myc to improve mesenchymal-to-endothelial transition and myocardial repair of cardiac-resident mesenchymal stem cells

Fig. 8Fig. 8

Outcome of the implanted cMSCs. A and B, Donor cMSCs in the infarcted hearts 30 days after transplantation. To determine the survival of implanted cMSCs (male) in infarcted myocardium (female), the hearts were stained against SRY 30 days after transplantation. Representative microscopic images of the infarct zone: brown nuclear staining indicates transplanted, SRY-positive cMSCs, and blue nuclear staining indicates host cells. cMSCs were found at the residual viable myocardium and were abundant in the heart sections receiving cMSCs co-transfected with oec-Myc and oeOct4 (A red arrows). C The number of SRY+cMSCs presented in the infarcted hearts after 30 days of transplantation. D and E Double immunostaining with EGFP (green) and anti-vWF (vascular endothelial cytoplasm were stained red) showed that EGFP+ cells were gathered around blood vessels and were abundant in the heart sections treated with oec-Myc plus oeOct4-transfected cardiac MSCs. Some of the transplanted EGFP+ cells were also stained with vWF (white arrows). F Number of capillaries measured by vWF positive capillaries per laminin-outlined cardiomyocyte in transverse heart Sects. 30 days after PBS injection or cell therapy. P value for the difference between groups was evaluated using Student’s t test. G Quantitative data of number of blood vessels in the infarcted hearts 30 days after PBS injection or cell therapy. C and G: P value for the difference between groups was calculated by the Welch test followed by a Brown-Forsythe multiple comparisons test. p < 0.05: *vs PBS injection, #vs. cMSCs therapy alone, Δvs. Transplantation of oec-Myc transfected cMSCs, vs. Transplantation of cMSCs transfected with oec-Myc plus oeOct4, §vs. Transplantation of cMSCs transfected with oec-Myc plus siOct4, vs. Transplantation of cMSCs transfected with sic-Myc, vs. Transplantation of cMSCs transfected with sic-Myc plus oeOct4 (n = 5, each group). H The correlation between the number of transplanted cells and the number of blood vessels in the oec-Myc + oeOct4 group. cMSCs indicate cardiac mesenchymal stem cells; SRY, sex-determining region Y; EGFP, enhanced green fluorescent protein; PBS, phosphate-buffered solution

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