Fig. 1

Insulin-resistant offspring of T2D patients and insulin-sensitive individuals generate pluripotent iPSCs. A M values indicating amount of glucose infused per min per kg of body weight as determined by hyperinsulinemic-euglycemic clamp for the recruited subjects. B Immunostaining for pluripotency markers for representative clones derived from each sample, C PCR for key pluripotency genes in IS and IR iPSCs, D karyotyping analysis of representative IS and IR iPSCs showing normal number of chromosomes. OCT4, green; SOX2, green; NANOG, green; TRA-60, red; TRA-81, red; SSEA-4, red. Scale bars = 100 µm