Fig. 1
From: Muse cells: ushering in a new era of stem cell-based therapy for stroke
Schematic diagram of the growth process of Muse cells. Muse cells could be isolated from MSC/fibroblast populations by MACS or FACS. In the suspension state, single Muse cells proliferate by asymmetric division and generate both Muse and non-Muse cells. The non-Muse cells enwrap Muse cells, and the enwrapped Muse cells grow gradually from a small-scale cluster to a 50–150 μm mature cluster in 10 days. When the mature cluster is transferred to adherent culture, inner Muse cells migrate out of the cluster and proliferate by asymmetric division. After repeated subculture, the proportion of non-Muse cells accounts for a proportion of cells consistent with that found in MSC/fibroblast populations. Abbreviations: A-Day 2/4/14, adherent culture for 2/4/14 days; FACS, fluorescence-activated cell sorting; MACS, magnetic-activated cell sorting; MenSCs, menstrual blood-derived endometrial stem cells; MSCs, mesenchymal stem cells; S-Day 1/3/7/10, suspension culture for 1/3/7/10 day. Scale bar: 100 μm in a and c-h; 50 μm in b.