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Fig. 2 | Stem Cell Research & Therapy

Fig. 2

From: ADSCs stimulated by VEGF-C alleviate intestinal inflammation via dual mechanisms of enhancing lymphatic drainage by a VEGF-C/VEGFR-3-dependent mechanism and inhibiting the NF-κB pathway by the secretome

Fig. 2

Characterization and identification of isolated ADSCs and VEGF-C stimulated ADSCs in vitro and homing of ADSCs in vivo. a Images of ADSCs in the primary passage on Day 1 and Day 3. Scale bar 100 μm; ×100, magnification. b, c Flow cytometry analysis of surface immunophenotype in the ADSCs and VEGF-C pretreated ADSCs, respectively. The blue line represents blank control and the red line represents marked ADSCs. d Detection of the multipotent capacity of ADSCs to induce adipogenic and osteogenic differentiation by Oil Red O and Alizarin Red staining, respectively. Scale bar 100 μm; × 200, magnification. e Images of ADSCs unstimulated and stimulated with VEGF-C at the 5th passage. Scale bar 100 μm; × 100, magnification. f The distribution of the injected PKH67-labelled ADSCs (green) in the murine colon under a fluorescence microscope with DAPI (blue). Scale bar 100 μm; × 200, magnification

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Stem Cell Research & Therapy

ISSN: 1757-6512