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Fig. 4 | Stem Cell Research & Therapy

Fig. 4

From: Generation of corrected hiPSC clones from a Cornelia de Lange Syndrome (CdLS) patient through CRISPR-Cas-based technology

Fig. 4

Karyotype, copy number variation (CNV) and precision of the CRISPR-Cas9 mediated NIPBL c.5483G > A correction in hiPSC-cl1. A Karyotype analysis conducted by carrying out a shallow Whole Genome Sequencing (sWGS) in hiPSCs-c.5483G > A. B Karyotype analysis conducted by carrying out a sWGS in hiPSCs-cl1. C CNV profile obtained by sWGS in hiPSCs-c.5483G > A. D CNV profile obtained by sWGS in hiPSCs-cl1. E GUIDE-seq analysis of gRNA + 4. F Sanger sequence of the top one predicted off-target of gRNA + 4 in hiPSCs-cl1. The protospacer is highlighted in black, PAM in red

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Stem Cell Research & Therapy

ISSN: 1757-6512