Fig. 4

Effects of hUC-MSC-CM on ROS generation and cell migration in UVB-irradiated HaCaT cells. a ROS production. Images on the left display results under fluorescence microscopy. The graphs on the right represent the fluorescence result statistics. The horizontal axis of the graph on the right represents different groups, whereas the vertical axis represents the relative fluorescence intensity. The above statistical graph results from the cell count, and the following are the statistical results of ROS. **, p < 0.01; *, p < 0.05. b Cell scratch assay. The left side displays images of cell scratches under the microscope, the horizontal represents different groups, and the vertical represents different times (0, 15, 30 h). The grey part indicates overgrown cells, and the green part represents the scratch condition. The panel on the right displays the statistical results of the scratch convergence. The extent of scratch closure was expressed as the wound confluence. Statistical results were expressed as the mean ± standard deviation (n = 3)