Fig. 3

Schematics of experimental design for assessments of gene/miRNA interactions and proliferation–maturation axis of D15 on hiPSC-CMs TROPO-GFP. A Proliferation and maturation plot combined (EdU-positive cardiomyocytes vs. TNNI3, MYH6, and MYH7 gene expression) of the differentiation time of hiPSC-CMs. The red box between D8-D15 and green box D23-D30 indicates the time windows used to validate miRNAs effects in the proliferation/maturation axis. B Schematic overview of the study. C Expression levels of possible gene/miRNAs interaction accessed on D11 or D30 after target-miRNAs mimics on hiPSC-CMs (N = 3–4 independent differentiation experiments). D Schematic to represent miRNAs present in Profiles 1 and 2 used in the concordance score for assessment of proliferation and maturation predictors, respectively, with expected outcome for mimic transfection given each profile characteristics. E hiPSC-CM proliferation estimated using the EdU assay. Results were plotted as the percentage calculated by the number of cTnI cells with EdU-positive nucleus versus all the cTnI positive nucleus. F Structural shift analysis based on cell mean fluorescence intensity of alpha-myosin heavy chain (MYH6 gene) and beta-myosin heavy chain (MYH7 gene) for each experimental group. Statistical significance treatment vs control (Ctrl) are represented as * p < 0.05, ** p < 0.001, *** p < 0.001. N = 3–4 independent differentiation experiments