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Fig. 5 | Stem Cell Research & Therapy

Fig. 5

From: Priming mesenchymal stem cells with α-synuclein enhances neuroprotective properties through induction of autophagy in Parkinsonian models

Fig. 5

Exosomes derived from primed MSCs are packed with autophagy-associated miRNA. A MSC and primed MSC-derived exosomes, diameter 50–500 nm, as visualized by EM and PTA staining at 20 K × , 50 K × , and 5 K × magnifications. Scale bar indicates the size of the field shown. B, C Characterization of exosomes using NTA and SP-IRIS immunophenotypic analysis. NTA was performed to measure the total number of particles (B) and particle distribution (C). D Comparison of miRNA in MSCs and primed MSC-derived exosomes. Heatmap shows unsupervised hierarchical clustering of samples, generated with Multi-Experiment Viewer (MeV v4.9). EG Quantitative RT-PCR analysis for autophagy-related miRNA shows that miR-200-3pb, miR1299, and miR-7-5p expression levels increase in α-syn-treated MSCs compared to those in control MSCs, but the change in miR-200b-3 pp expression is not significant. H Network analysis with IPA shows that miR-7-5p miR-1299a, and miR-200b-3p targeted genes are involved in AMBRA1-induced autophagy cascade

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