Fig. 7

MSC priming exerts neuroprotection in α-syn-overexpressing mice. A TH-positive neuron counting in the SN at 4 weeks reveals that naïve and primed MSC treatment induces higher numbers of dopaminergic neurons compared to the α-syn-overexpressing control MSC group. B Western blotting shows that both naïve and primed MSC treatment significantly increases TH expression levels compared to the α-syn-overexpressing control MSC group. C Western blotting for autophagy markers shows that naïve and primed MSC treatments significantly upregulate autophagy-related markers, LC-3, Beclin-1, and AMBRA1 compared to the α-syn-overexpressing control MSC group. D Both naïve and primed MSC treatments lead to an increase in the expression of autophagy flux-related marker p-Akt/Akt and E lysosome activity markers, LAMP 1 and TFEB, compared to the control MSC group. The levels of target protein were quantified and normalized to β-actin using ImageJ. Data are presented as mean ± SE. *P < 0.05; **P < 0.01