Fig. 3

RPE frozen at P2D5 maintained the biological function after thawing. A Quantification analysis of PEDF secreted by thawed RPE cells frozen at indicated time points. PEDF secretion levels were measured by ELISA at D28 post thaw for both apical and basal compartments of transwell culture. B TEER of RPE cells at D28 post thaw. C Quantification analysis of relative fluorescence of 488-POS bound and ingested by RPE cells at D28 post thaw. n = 3 biological replicates. Data are represented as mean ± SD. Statistical differences are evaluated with two-way ANOVA with Bonferroni's post hoc test (A) or one-way ANOVA with Tukey's post hoc test (B, C). *P < 0.05