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Fig. 5 | Stem Cell Research & Therapy

Fig. 5

From: Synovial membrane-derived mesenchymal progenitor cells from osteoarthritic joints in dogs possess lower chondrogenic-, and higher osteogenic capacity compared to normal joints

Fig. 5

Evaluation of osteogenic differentiation by Alizarin Red staining. A Calcium (stained in red) and the presence of noduli are representative of successful osteogenic differentiation. Representative images of every donor were obtained using the brightfield setting of an Olympus IX51 inverted microscope. The negative controls received expansion medium for 21 days. The scale bar is set at 500 µm. The donor numbers of clinically normal (N) and OA (O) donors correspond to the donor numbers and information in Additional file 1: Table S1. B The percentage (%) of Alizarin Red positive area was measured using Image J (Fiji) software in at least 3 image per donor per condition. C Gene expression of the osteogenic markers alkaline phosphatase (ALP) and RUNX2 is shown as the N-fold change of the cells treated with osteogenic differentiation medium compared to their own negative control. Each dot represents an individual donor

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Stem Cell Research & Therapy

ISSN: 1757-6512