Fig. 2
From: Connective tissue growth factor promotes cementogenesis and cementum repair via Cx43/β-catenin axis
CTGF promoted cementogenesis of hPDLSCs in vitro. A ARS staining showed a greater increase in mineralized nodules in hPDLSCs treated with the ascending-concentration of CTGF relative to the control group on the 14th day. B ALP staining on day 7 demonstrated the enhanced ALP activity in hPDLSCs after CTGF treatment. C Representative WB results of the mineralization-related proteins showed increasing expression of Osx, Runx2, CAP and Col1 in hPDLSCs when stimulated with the concentration-ascending CTGF on the 14th day. D–G Representative charts indicating the quantification of Osx, Runx2, CAP and Col1 in Fig. 3C. n = 3. Data are obtained from three independent images. *p < 0.05. H–L qPCR data showed that the expression of mineralization-related factors (Runx2, Osx, CAP, CEMP, ALP) enhanced in hPDLSCs treated with the concentration-ascending CTGF on the 14th day. Data are obtained from three independent results. *p < 0.05