Fig. 4

CTGF accelerated the Cx43 accumulation in time and dose-dependent manners in vitro. A Western blotting illustrated that CTGF accelerated the Cx43 accumulation in a concentration-dependent manners for 12 h. β-actin acted as the reference for quantification. B Western blotting illustrated that CTGF accelerated the Cx43 accumulation in a time-dependent manners for 12 h. β-actin acted as the reference for quantification. C, D Quantification of Cx43 proteins induced by CTGF (50 ng/ml) in Fig. 5A, B. Statistical significance is indicated. *p < 0.05. E IF results showed the dynamic distribution of Cx43 expression over time, and CTGF accelerated the accumulation of Cx43 at cell junctions, which manifested as red fluorescent plaques