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Fig. 3 | Stem Cell Research & Therapy

Fig. 3

From: Hsa_circ_0001485 promoted osteogenic differentiation by targeting BMPR2 to activate the TGFβ-BMP pathway

Fig. 3

Hsa_circ_0001485 bound to BMPR2 to activate the BMP signaling pathway in the osteogenic hFOB 1.19 cells. A Design of the pattern diagram of the hsa_circ_0001485 probe for the pull-down test. B Silver staining analyzed the pull-down products. C The target protein BMPR2 was identified by mass spectrometry. D The western blot detected the expression of BMPR2 and its downstream proteins Smad1/5/9, p-Smad1/5/9, and Smad4. E, F Quantitative analysis of BMPR2, Smad1/5/9, p-Smad1/5/9, and Smad4 was performed by densitometry. The results represent data from three replicates, and the mean ± SD (**P < 0.01)

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Stem Cell Research & Therapy

ISSN: 1757-6512