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Fig. 4 | Stem Cell Research & Therapy

Fig. 4

From: Exopolysaccharide of Enterococcus faecium L15 promotes the osteogenic differentiation of human dental pulp stem cells via p38 MAPK pathway

Fig. 4

L15 EPS enhances osteogenic differentiation of hDPSCs. A Photomicrograph of ALP staining of hDPSCs with or without L15 EPS. Inverted imaging system (EVOS™ XL Core Imaging System; Thermo Scientific™, Waltham, MA, USA). Scale bar = 200 μm B Photograph showing calcium deposition of hDPSCs with or without L15 EPS by ARS staining. C The expression of osteogenic differentiation marker genes (RUNX2, ALP, and COL1A1) were analyzed by real time-PCR. D The expression of osteogenic differentiation marker proteins (BMP4, RUNX2, and ALP) expression were analyzed by western blot analysis. (E) Quantitative analysis of intensity in (D) relative to GAPDH. Scale bar = 200 μm. *p < 0.05, N = 3

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