Fig. 4

Evaluation of the MSC-like properties in the Pooled Clones CLP lip fibroblasts. a Diagram of the strategy used to obtain the Pooled Clones CLP (Pooled C. CLP) population. b qPCR (left) and FACS (right) analysis for the expression of the MSC-surface markers CD106 and CD146 in BM-MSCs, the parental CLP and the Pooled Clones CLP population. Black dashed lines in the FACS plots indicate the threshold of unstained samples. The percentage of positive cells for each sample is reported. Gating strategy is presented in Additional file 1: Fig. S6. *p < 0.05 BM-MSCs versus CLP or Pooled Clones CLP. c Brightfield images after ARS (day 21) and CV staining (day 14) show, respectively, mineralization and single-cell derived colonies in BM-MSCs, parental CLP and Pooled Clones CLP populations. Histograms to the right report quantification of the ARS staining by ImageJ and quantification of the colonies. Scale bars: 5 µm (ARS); 1 cm (CV). *p < 0.05 BM-MSCs versus CLP or Pooled Clones CLP. a.u.: arbitrary unit