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Fig. 4 | Stem Cell Research & Therapy

Fig. 4

From: Translational development of ABCB5+ dermal mesenchymal stem cells for therapeutic induction of angiogenesis in non-healing diabetic foot ulcers

Fig. 4

Blood flow recovery and neovascularization following surgically induced HLI in OF1 mice. A Representative LDPI acquisition before and immediately after HLI induction, illustrating the experimental setup. Scanned areas are marked by ellipses; the warmest color (intense red) represents 200 perfusion units. Graphs show mean perfusion unit during 1 min in the non-ischemic (blue) and ischemic (red) thigh. B LPDI ratio between the ischemic and the non-ischemic limb in mice treated with 5 × 106 ABCB5+ MSCs or vehicle. Means with SD of n = 10 (day 1), n = 9 (day 3), n = 8 (day 5; days 7–21 MSCs) and n = 7 (days 7–21 vehicle) animals. C–F Immunohistochemical and histopathological evaluation of the ischemic hindlimb muscles at 6 days after HLI induction in mice treated with ABCB5+ MSCs or vehicle injected into the ischemic limb 24 h after surgery. C CD31 expression in the thigh muscles, presented as mean (SD) IHC score, with 0 = none, 1 = minimal, 2 = slight, and 3 = moderate, of n = 12 animals. D Representative H&E sections of the gastrocnemius muscle from a vehicle- and an MSC-treated mouse, showing inflammatory and degenerative lesions in both mice and increased neovascularization in the MSC-treated mouse. Scale bars: 50 µm. E Degenerative and inflammatory processes in the gastrocnemius muscle, presented as mean (SD) summary score according to ISO 10993–6:2007 of n = 6 (vehicle) and n = 7 (MSCs) animals. F Neovascularization in the gastrocnemius muscle, presented as mean (SD) score, with 0 = none, 1 = 1–3 focal buds, 2 = groups of 4–7 capillaries with supporting fibroblastic structures, 3 = broad band and 4 = extensive band of capillaries with supporting fibroblastic structures, of n = 6 (vehicle) and n = 7 (MSCs) animals. *p < 0.05, **p < 0.01, ***p < 0.001 versus baseline (B) or vehicle (C, E, F); one-way ANOVA with Dunnett’s post hoc test

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