Fig. 2

The primed hADSCs were more effective than untreated hADSCs to alleviated TNBS-induced colitis and colonic fibrosis. (A) Experimental layout of primed and untreated hADSCs treatment in the acute colitis and chronic colon fibrosis; (B) Therapeutic efficacy evaluation of acute TNBS model. a. endoscopy observed on the 7th day. b. body weight change percentage (n = 5). c. anatomic observed on the 10th day. d. DAI scores (n = 5); (C) body weight change percentage (n = 5); (D) DAI scores (n = 5); (E) endoscopy observed on the 7th and 40th day; (F) rats were killed at the 7th week, the length of the colon was counted and the lesions of the colon were observed; (G) H&E staining was performed to observe the structural integrity of intestinal and the infiltration of immune cells in each group and score (Magnification 4X and 10X; Scale bar = 200, 100 μm; n = 5); (H) MPO activity detected (n = 5); (I) qPCR detection mRNA expression changes of inflammatory cytokines IL-1β, TNF-α, IL-6 and anti-inflammatory molecule IL-10 (n = 5); *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001. Data are represented as mean ± SEM