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Fig. 4 | Stem Cell Research & Therapy

Fig. 4

From: The role of DNA demethylation in liver to pancreas transdifferentiation

Fig. 4

Downregulation of DNMT1 expression promotes pTFs induced TD: DNMT1 expression was knocked-down using specific shRNAs (sh#4 and sh#5) delivered by GFP-expressing lentiviruses, as described in materials and methods: lentivirus infection efficiency was measured by A GFP expression of treated cells at 2 weeks post lentiviral infection. B mRNA levels of DNMT1 at 2 weeks post lentiviral infection. N = 8 p < 0.000003. C Western blot analysis of DNMT1 protein levels in 2 selected cell lines at 2 weeks post infection. D Protein level quantification of DNMT1 at 2 weeks post lentiviral infection, compared to scrambled shRNA delivery. N = 4, p < 0.0006. Stable clones of DNMT1 knocked down liver cells were transdifferentiated by ectopic expression of pTFs. The expression levels of pancreatic specific TFs (E) and pancreatic specific genes (F) was measured 6 days post TD induction by RT-qPCR. Results are presented as average ± SD, n = 4 different donors, *p < 0.01, **p < 0.05

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