Fig. 6
From: The role of DNA demethylation in liver to pancreas transdifferentiation
TD-prone cells display basal DNA hypomethylation compared to TD-resistant liver cells and further undergo demethylation upon pTF-induced TD A Heat map of 6017 CpGs with over 20% differences in resistant (n = 4) and prone cells (n = 6), adjP < 0.05. B Boxplot display of 6017 over 20% DMPs in resistant (n = 4) vs. prone (n = 6) human liver primary cell lines, adjP < 0.05. TD-prone cells underwent the TD protocol, and average global methylation across all probes in Infinium HumanMethylation450 BeadChip Kit was determined (Illumina). C Overall methylation profile of viral control (β-gal) infected cells, untreated cells (untouched), and TD cells. Red lines indicate the average methylation level. D Methylation profile of altered meCgGs. Compared to untreated cells, 265 CpG’s methylation levels were significantly altered in TD cells