Fig. 7
From: The role of DNA demethylation in liver to pancreas transdifferentiation
Significant increase in TD efficiency occurs upon combination of pTFs with epigenetic modifying agents. Liver cells were treated with a combination of soluble factors, as described in the materials and methods section, and infected 48 h later with pTFs. Expression of the pTFs NKX6.1,PAX6, and ISL-1 as well as the pancreatic specific genes GCG, SST and SCG2was analyzed by RT-qPCR. Results are presented as average ± SD, n = 3 different donors, *p < 0.01, **p < 0.05 related to TD alone, ##p < 0.01, #p < 0.05 related to TD + LiCl + 5-AZA + SAHA