Skip to main content
Fig. 9 | Stem Cell Research & Therapy

Fig. 9

From: Integrative network analysis of circular RNAs reveals regulatory mechanisms for hepatic specification of human iPSC-derived endoderm

Fig. 9

hsa_circ_004658/miR-1200/CDX2 axis suppresses the Wnt/β-catenin signalling pathway, as determined by western blotting and immunofluorescence. A The putative binding site between miR-1200 and CDX2. B Validation of CDX2 as a miR-1200 target gene by dual-luciferase reporter assay. C Relative miR-1200 expression in the hsa_circ_004658 and pLC5-ciR groups. D Fluorescence intensities of the markers (β-catenin, p-β-catenin and TCF4) of the Wnt/β-catenin signalling pathway and CDX2 among the hsa_circ_004658, pLC5-ciR and control groups. E The fluorescence signal of β-catenin was captured in hsa_circ_004658 and pLC5-ciR groups cells (100×). F Fluorescence intensities of the markers (β-catenin, p-β-catenin and TCF4) of the Wnt/β-catenin signalling pathway after CDX2 overexpression. Relative optical density analysed by Image J. The experiments were performed three times. *p < 0.05, **p < 0.01; ns, not significant

Back to article page