Table 1 PCR Primer lists
circRNAs | Primer sequence | Annealing temperature (℃) | Length (bp) |
|---|---|---|---|
GAPDH(HUMAN) | F:5′GGGAAACTGTGGCGTGAT3′ R:5′GAGTGGGTGTCGCTGTTGA3′ | 60 | 299 |
hsa_circRNA_004658 | F:5′ GCCATCAGCAAATAATGAACCCA3′ R:5′ CGTCCTTGTGAGTCGAAGAACCT3′ | 60 | 169 |
hsa_circRNA_075671 | F:5' ACCAGCATCAGAACAGTCAGAGAA3' R:5′ ACCACGGAATCCCATCACTGT3′ | 60 | 133 |
hsa_circRNA_006919 | F:5′ CAAACTCGTGGCAATATGTG3′ R:5′ CTGCCCAGTCCAGTCCTAT3′ | 60 | 285 |
hsa_circRNA_104101 | F:5′ GGAGCAACAGTAGAAAGTTCTCTA3′ R:5′ GGCACCTTCATCAGTAGTCATT3′ | 60 | 56 |
hsa_circRNA_104981 | F:5' GCGCTATGACAAATTGAAGATA3' R:5′ TTCTGACTCCAGTGTTTCCATC3′ | 60 | 96 |
hsa_circRNA_102700 | F:5' CCAGAATGATGAAATTGTTAGGT3' R:5′ TAATCGCCGCATGTTGTAC3′ | 60 | 63 |
hsa_circRNA_005232 | F:5′ ATCGTTCCATATAAAACCATCG3′ R:5′ CTTCCAACTGTCACAACACAAT3′ | 60 | 115 |
hsa_circRNA_002415 | F:5′ GGAGCATGAGGATGAGAAGATA3′ R:5′ CAGTTCTGACTCCAGTGTTTCC3′ | 60 | 99 |
hsa_circRNA_104730 | F:5′ AAGCCAAAGCAGCAGGAGTT3′ R:5′ GGTGTGGGTTATAAGCCTTTCC3′ | 60 | 68 |