Fig. 1

Cytotoxicity of NAC at various concentrations in hDFSCs. A Immunofluorescent staining of cytoskeleton structure (green for phalloidin, blue for DAPI) at day 3 after treatment. Scale bars: 50 μm. B Cell viability detected by CCK-8 assay at 24, 48 and 72 h. C Cell cycle picture and distribution detected by flow cytometry. D Apoptosis picture and the distribution of early and late apoptotic and viable cells detected by flow cytometry. E Photomicrographs and the distribution of blue-stained senescent cells of passage 12 using SA-β-Gal staining. Scale bars: 100 μm. F Relative mRNA expression of p21 and p53. G The distribution of aneuploid cells detected by flow cytometry. Statistically significant differences between groups were determined by P < 0.05 (*), P < 0.01 (**), P < 0.001 (***), P < 0.0001 (****)