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Fig. 4 | Stem Cell Research & Therapy

Fig. 4

From: Effects of hypoxia-preconditioned HucMSCs on neovascularization and follicle survival in frozen/thawed human ovarian cortex transplanted to immunodeficient mice

Fig. 4

TUNEL-positive stroma cells and follicles. A percentage of TUNEL-positive apoptotic stroma cell, B percentage of TUNEL-positive apoptotic follicle, data were represented as mean ± SEM and analysed by one-way ANOVA and Fisher’s LSD post hoc test. C TUNEL staining of non-grafted and grafted tissues in the OT, N-MSCs + OT, and H-MSCs + OT groups on day 3. Green fluorescence: TUNEL-positive nuclei; blue fluorescence: DAPI. White arrows: TUNEL-positive. Scale bars: 50 μm. P value references: *P < 0.05; **P < 0.01; ***P < 0.001. Statistical significance is also expressed using different letters, a vs. b P < 0.05. TUNEL, terminal deoxynucleotidyl transferase dUTP nick-end labelling; OT, ovarian tissue transplantation; N-MSCs + OT, normoxic-treated HucMSCs, and ovarian tissue co-transplantation. H-MSCs + OT, hypoxia-treated HucMSCs, and ovarian tissue co-transplantation. n = 4 per group and per time point

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