Fig. 5

ADCP in BMDMs was enhanced by sensitized serum. Representative FCM results are shown in (A). M0: Medium only; M1: LPS + IFN-γ stimulation 2, M2: IL-4 stimulation. A GFP-positive cells in singlets (FSC vs. SSC) and CD11b-positive fraction of cells collected after 24 h of co-culture. The percentage of GFP-positive cells in the CD11b-positive fraction is shown (B). The t test between normal and sensitized sera within the corresponding BMDM activation phenotypes. Data are expressed as the mean ± S.E. (n = 4, each phenotype). There were significant differences between normal and sensitized sera in M0, M1 and M2 phenotypes. There were no differences among the sensitized groups (M0, M1, and M2) or normal groups. *p < 0.05, **p < 0.01, ***p < 0.001