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Fig. 2 | Stem Cell Research & Therapy

Fig. 2

From: Implantation of adipose-derived mesenchymal stem cell sheets promotes axonal regeneration and restores bladder function after spinal cord injury

Fig. 2

Preparation of ADSC sheets. a ADSCs were observed by light microscopy. b Oil Red staining revealed lipid droplet aggregation in the cytoplasm of ADSCs. c Alizarin Red staining revealed calcium deposition in ADSCs. d ADSC sheet formation after 14 days of culture. e Light microscopic view of ADSC sheet morphology. f SEM of an ADSC sheet. g TEM showing tight junctions in the ADSC sheet. h TEM showing the ECM in the ADSC sheet. i TPEF images showing collagen bundles in the ADSC sheet. Scale bars: 100 μm (a, b, c, e); 50 μm (f); 20 nm (g, h); 20 μm (i)

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