Fig. 5

Cryopreserved MSC overexpressing CD::UPRT::GFP exerted strong anti-cancer effect against multiple cancer cell lines. Freshly modified or cryopreserved modified MSCs were co-cultured separately with (A) A549, (B) CLAC, (C) Hs 888.T and (D) RPMI 2650. MSC overexpressing CD::UPRT::GFP were added at a ratio of 1 MSC to 1, 5 or 10 cancer cells and subsequently, 150 µg/mL 5FC was added to the wells. After six days, a standard MTS assay was used to determine the cell viability and killing. Conditions without 5FC treatment serve as the controls. (E) Representative bright field images of the (1 MSC:1 cancer cell) mixed cultures at the end of the experiment prior to MTS assay. The graph represents data from technical quintuplicates (n = 5) presented as mean + SD. Significant differences of the killing capacity between freshly modified and cryopreserved modified MSCs were calculated using two-tailed Student’s t test. *P < 0.05, **P < 0.005