Fig. 5From: Human umbilical cord mesenchymal stromal cell-derived exosomes protect against MCD-induced NASH in a mouse modelA. Immunohistochemical assays were used to detect distribution and location of PPARĪ± in groups of mouse livers. (a) Regular chow; (b) MCD group; (c) MCD with Control Medium (CM) extract intervention group; (d) MCD with hUC-MSC exosomes(Ex) group. The random view to zoom in on the panel shown is on the right. Scale barā=ā20Ā Ī¼m. B. Western blotting analysis of PPARĪ± in liver tissues in groups of mice. Three samples were selected in each group (upper panel). Protein levels were quantified using ImageJ software and are represented by a histogram (fold change of PPARĪ±/GAPDH, panel below). Full-length blots are presented in Additional file 1: Fig. S3. C. PKH-26 labeling of MSC-exo taken up by HepRG cells. Scale barā=ā20Ā Ī¼m. D. PKH-26 labeling of MSC-exo taken up by Huh1-6 cells. Scale barā=ā20Ā Ī¼m. E. Western blotting analysis of PPARĪ± in the human liver cell line HepRG under the indicated treatment. Protein levels were quantified using ImageJ software and represented by a histogram (fold change of PPARĪ±/Histone H3). **pā<ā0.01. Full-length blots are presented in Additional file 1: Fig. S4. F. Western blotting analysis of PPARĪ± in the mouse hepatoma carcinoma cell line Huh1ā6 under the indicated treatment (fold change of PPARĪ±/Histone H3). Protein levels were quantified using ImageJ software and represented by a histogram. **pā<ā0.01. Full-length blots are presented in Additional file 1: Fig. S5Back to article page