Fig. 1
From: Stability and biosafety of human epidermal stem cell for wound repair: preclinical evaluation
Preparation and identification of EpiSCs from the human prepuce. A The method of preparing EpiSCs from the human prepuce. B HE staining results of the prepuce (under 10 × optical microscopy, the scale was 200 μm). C Morphology of cultured cells (P1) (under 20 × optical microscopy). D Flow cytometry detection of cultured cells (P1). Q3 indicated that the ratio of high CD49f expression to low CD71 expression was approximately 90.5%. (E, F, G) Immunofluorescence staining of cells (P1). Blue indicates DAPI, red indicates integrin β1 (Cy3), and green indicates CK19 (Fluor488) (under 40 × confocal microscopy)