Table 3 Genotyping primers for MKX-tdTomato reporter
From: Establishment of a human pluripotent stem cell-derived MKX-td Tomato reporter system
Purpose | Name | Sequence |
|---|---|---|
Knock-in check(WT: 2130Â bp, KI: 3037Â bp) | MKXreporter_OUT(F) | CCCTGACATTGTGGGAGGTC |
MKXreporter_OUT(R) | ACTGGCTGCACTATTGACCC | |
Cloning primers for 5′ boundary | out-F(1) | TGCAATTACAGAAACCCACCA |
ires-R(1) | CACACCGGCCTTATTCCAAG | |
Cloning primers for 3′ boundary | PGK-F(2) | CCAGACTGCCTTGGGAAAAG |
out-R(2) | ACCAGAGCTCAGGCTCCAAA |