Fig. 1
From: Regulatory of miRNAs in tri-lineage differentiation of C3H10T1/2
The biogenesis and function of miRNA processing. The maturation of miRNAs includes the production of primary miRNA transcripts (pri-miRNA) by RNA polymerase II or III and cleavage of pri-miRNA in the nucleus by the microprocessor complex Drosha-DGCR8 (Pasha). The resulting precursor hairpin, pre-miRNA, is exported from the nucleus via exportin-5-rang-gtp is exported from the nucleus. In the cytoplasm, the functional strand of the mature miRNA is loaded with Argonaute (Ago2) protein into the RNA-induced silencing complex (RISC), where it directs RISC to silence the target mRNA by mRNA cleavage, translational repression or deadenylation, while the passenger strand is degraded