Table 5 Modulation of MAPK signal pathway in tri-lineage differentiation of C3H10T1/2
From: Regulatory of miRNAs in tri-lineage differentiation of C3H10T1/2
Differentiation | Target | Expression | Expression of MAPK | Distribution | Function | Experiment | Sample | References |
|---|---|---|---|---|---|---|---|---|
Osteogenic differentiation | miR-122-5p | ↑ | ↓ | Exosomes | Overexpressed miR-122-5p inhibits the RTK/Ras/MAPK signaling pathway, thereby down-regulating SPRY2 and promoting osteogenesis | In vivo and in vitro | Mice/80 | [101] |
miR-7 | ↓ | ↑ | Cytoplasm | The down-expression of miR-7 up-regulated GDF5, promoting the phosphorylation of Smad1/5/8 and p38 MAPK to facilitate osteogenic differentiation | In vivo | – | [102] | |
miR-503 | ↑ | ↓ | Cytoplasm | miR-503 down-regulate RANK to suppress the MAPK and AKT pathways, which resulted in decreased expression of osteoclastogenesis-related markers | In vivo | – | [96] | |
miR-103 | ↑ | ↓ | Cytoplasm | miR-103 inhibit the activation of mechanical kinase ROCK1 and p-Erk1/2 in the MAPK signaling pathway, thereby inhibiting the classical osteogenesis-related Wnt/β-catenin pathway | In vivo and in vitro | Mice/40 | [103] | |
miR-210-3p | ↓ | ↑ | Nucleus | miR-210-3p suppresses osteogenic differentiation through targeting KRAS and suppressing the MAPK signaling; KRAS overexpression could reverse the suppressive effects of miR-210-3p overexpression | In vivo | – | [104] | |
miR-193a-3p | ↑ | ↓ | Cytoplasm | miR‐193a‐3p binds to Map3k3 mRNA, which results in down-regulation of Map3k3 expression, suppressing bone formation | In vivo and in vitro | Mice/60 | [105] | |
Adipogenic differentiation | miR-143 | ↑ | ↓ | Cytoplasm | The overexpression of miR-143 blocks the MAP2K5 ERK5 signaling pathway, so the ERK5-mediated phosphorylation of PPAR-γ is reduced | In vivo | – | [98] |
miR-21a-5p | ↑ | ↓ | Nucleus | miR-21a-5p inhibited BPA induced adipocyte differentiation by targeting map2k3 through MKK3/p38/MAPK | In vivo | – | [106] | |
miR-21 | ↓ | ↑ | Cytoplasm | miR-21 modulated ERK-MAPK signaling activity by repressing SPRY2 expression, a known regulator of the receptor tyrosine kinase (RTK) signaling pathway, to affect the duration and magnitude of ERK-MAPK activity | In vivo | – | [88] | |
Chondrogenic differentiation | miR-23a-3p | ↓ | ↑ | Cytoplasm | miR-23a-3p regulated by lncRNA SNHG5 suppresses the chondrogenic differentiation via targeting activating the JNK/MAPK/ERK pathway | In vivo | – | [107] |
miR-320 | ↓ | ↑ | Cytoplasm | miR-320 promoted the activity of a reporter construct containing the 3'-UTR and inhibited MMP-13 expression | In vivo | – | [100] | |
miR-125b | ↓ | ↓ | Nucleus | The down-regulation of miR-125b induced apoptosis and suppress other differentiation via BMPR2 | In vivo and in vitro | Mice/40 | [108] |