Table 7 Modulation of BMP signal pathway in tri-lineage differentiation of C3H10T1/2
From: Regulatory of miRNAs in tri-lineage differentiation of C3H10T1/2
Differentiation | Target | Expression | Expression of BMP | Distribution | Function | Experiment | Sample | References |
|---|---|---|---|---|---|---|---|---|
Osteogenic differentiation | miR-30a | ↑ | ↓ | Cytoplasm | The overexpression of miR-30a led to expression of an early osteogenic marker and a reduction in Runx2 expression, thus inhibiting the osteogenic differentiation of C3H10T1/2 | In vitro and in vivo | Mice/24 | [45] |
|  | miR-125b | ↑ | ↓ | Cytoplasm | The over and interferential expression of miR-125b down-regulate for Cbfβ protein in C3H10T1/2 cells and the overexpression decrease the mRNA levels of three osteoblastic marker genes, ALP, OCN, OPN by BMP-2-induced | In vitro and in vivo | Mice/40 | [53] |
|  | miR-214-3p | ↑ | ↓ | Nucleus | miR-214-3p could prevent C3H10T1/2 osteogenic differentiation by targeting BMP-2 and blocking BMP-TGFβ axis | In vitro | – | [146] |
|  | miR-128 | ↑ | ↓ | Cytoplasm | The overexpression of miR-128 promoted osteogenic differentiation of C3H10T1/2 by up-regulating ALP, matrix mineralization, mRNA, and protein levels of BMP-2 | In vitro | – | [147] |
Adipogenic differentiation | miR-669a-5p | ↑ | ↓ | Cytoplasm | The overexpression of miR-669a-5p promotes adipogenic differentiation and causes browning of C3H10T1/2 cells | In vitro | – | [148] |
Chondrogenic differentiation | miR-127-5p | ↑ | ↓ | Cytoplasm | LncRNA DNM3OS regulates GREM2 via miR-127-5p to suppress early chondrogenic differentiation of C3H10T1/2 under hypoxic conditions | In vitro | – | [149] |
|  | miR-199a | ↑ | ↓ | Cytoplasm | miR-199a inhibits Smad1/Smad4-mediated transactivation of target genes, and that overexpression of Smad1 completely corrects miR-199a-mediated repression of early chondrogenesis | In vitro and in vivo | Mice/48 | [142] |
|  | miR-99a | ↑ | ↓ | Cytoplasm | miR-99a knockdown promoted proteoglycan deposition and increased the expression of ACAN and COL2A1 during early chondrogenic differentiation | In vitro | – | [150] |