Fig. 3

AA and GW increase iPSC-CM fatty acid metabolism and mitochondrial function gene expression. Combined relative gene expression of all three cell lines evaluated across multiple differentiation batches at D27. a PGC1A CTRL versus AA, GW, and T3, respectively: 0.22 (0.04–1.89) versus 0.22 (0.03–0.42, n.s.), 0.49 (0.25–1.85; p = 0.0005), and 0.71 (0.34–1.20; p < 0.0001), b LDHB CTRL versus AA, GW, and T3, respectively: 0.71 (0.23–1.91) versus 1.24 (0.3–3.90; p = 0.07), 1.09 (0.42–1.46, n.s.), and 0.93 (0.52–1.71; p = 0.06), c CPT1B CTRL versus AA, GW, and T3, respectively: 0.61 (0.37–1.54) versus 0.68 (0.33–1.08, n.s.), 1.62 (0.88–5.67; p < 0.0001), and 1.01 (0.40–2.68; p = 0.0008), and d IMMT CTRL versus AA, GW, and T3, respectively: 1.03 (0.69–1.35) versus 1.99 (1.45–3.70; p < 0.0001), 2.12 (0.77–3.81; p = 0.0005), and 4.43 (2.56–5.94; p < 0.0001). e Representative images of fluorescent double immunolabelling of iPSC-CMs (UKKi036-C D27) for cardiac troponin T (green) and ATP5A (red). Hoechst (blue) indicates nuclei. Scale bar: 100 µm