Fig. 1From: Effect of glucose depletion and fructose administration during chondrogenic commitment in human bone marrow-derived stem cellsInfluence of glucose and fructose on matrix deposition. Human BMSCs chondrogenic pellets were cultured for 21 days in the presence of different concentrations of glucose (25 mM, 5.5 mM and 1 mM), alone or in combination with 25 mM fructose. A Safranin-O/Fast Green staining on pellet cryosections, scale bar 1 mm. B Sulphated glycosaminoglycans content in pellets as determined by the DMMB assay. C DNA content in pellets. D Analysis of GAG production normalized to the DNA content (µg/µg). Data are expressed as mean ± SD (n = 3 BMSC donors), and the differences between each condition and the HG group were analysed with one-way ANOVA. a: p ≤ 0.0001; HG versus LG. b: p ≤ 0.0001; HG versus LLG. c: p ≤ 0.0001; HG versus FRU. d: p ≤ 0.0001. LG versus LLG. e: p ≤ 0.0001; LG versus HG + FRU. f: p ≤ 0.0001; LG versus LG + FRU. g: p ≤ 0.001; LG versus LLG + FRU. h: p ≤ 0.0001; LLG versus HG + FRU. i: p ≤ 0.0001; LLG versus LG + FRU. j: p ≤ 0.0001; LLG versus LLG + FRU. l: p ≤ 0.0001; LLG versus FRU. m: p ≤ 0.0001; HG + FRU versus FRU. n: p ≤ 0.0001; LG + FRU versus FRU. o: ≤ 0.001; LLG + FRU versus FRU. a: p ≤ 0.05; HG versus LLG; b: p ≤ 0.05; HG versus LLG + FRU. c: p ≤ 0.01; HG versus FRU. d: p ≤ 0.05; HG + FRU versus FRU. . a: p ≤ 0.05; LLG versus LLG + FRU b: p ≤ 0.05; LLG versus FRUBack to article page