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Fig. 2 | Stem Cell Research & Therapy

Fig. 2

From: Effect of glucose depletion and fructose administration during chondrogenic commitment in human bone marrow-derived stem cells

Fig. 2

The chondrogenic potential of BMSCs in different cell culture media evaluated by type-II collagen immunostaining. A Representative pictures of n = 3 donors, here donor #2 is represented. Pellet cryosections stained for Collagen type II. The intensity of the fluorescent secondary antibody is directly proportional to the production of different collagen content, while DAPI (blue) stains cell nuclei. Scale bar (bottom right in each picture) = 200 µm (5× magnification) and 100 µm (10×). B Mean intensity and area of type-II collagen positive pellets. Data are expressed as mean ± SD (*p ≤ 0.05, **p ≤ 0.01 vs HG group). N = 3 BMSC donors

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