Fig. 5

Analysis of GLUT3 and GLUT5 on naïve hBMSCs. A Representative image depicting flow cytometry analysis of GLUT3 and GLUT5 expression on naïve hBMSCs. The gating strategy included the first selection of cells based on the FSC/SSC dot-plot. After the selection of the hBMSCs population, the range of GLUT3 (green histogram) and GLUT5 (red) positivity was set based on the negative controls (staining with secondary antibody only, grey peaks in histogram overlays). B Analysis of GLUT3 (SLC2A3) and GLUT5 (SLC2A5) expression by qPCR. The graph represents the gene expression levels of the single donors performed in three technical replicates, while the table summarizes the expression ratios between the two genes (2^−ΔCt ratio)