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Fig. 2 | Stem Cell Research & Therapy

Fig. 2

From: Robust generation of human-chambered cardiac organoids from pluripotent stem cells for improved modelling of cardiovascular diseases

Fig. 2

Cardiovascular progenitor cells play an essential role in the self-organization of a chambered cardiac organoid. A Mixed, control population stained with SIRPα and EDNRA fluorescent antibodies. SIRPα single-positive cells (bottom right quadrant) contain the cardiomyocyte population, while the SIRPα, EDNRA double-positive cells (top right quadrant) contain the cardiovascular progenitor population. B Percentages of contracting and chambered cardiac organoids generated using different compositions of cell populations. DN Double Negative Cells; CM SIRPA+ EDNRA− Cardiomyocytes; CVP SIRPA+, EDNRA+ Cardiovascular Progenitor Cells (n = 6 per group). C Representative 2-Photon and brightfield images stitched from adjacent fields depicting CCOs generated using 50% CVPs and 50% CMs. CVPs were stained with Calcein AM (green). Scale bars, 500 µm. D Representative confocal images of CCOs generated using CVPs and CMs. CVPs were stained with Calcein AM (green) and Hoechst33342 (blue) and CMs were only stained with Hoechst33342. Scale bars, 500 µm

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