Fig. 6
Altered calcium transients are recapitulated in chambered cardiac organoids treated with CH inducer EDN1. A Illustration depicting how the various calcium transient parameters were derived per single cardiomyocyte contraction rhythm. B Representative calcium transient profile in control, 50 ng/ml and 100 ng/ml EDN1-treated H7-GCaMP6s-derived CCOs. C–E Dot plot showing mean beat-to-beat duration (s), mean calcium transient duration (CTD) (s), mean depolarization duration (s), and repolarization duration (30th, 60th, 90th per cent) across cells analysed for 1 week (C) 2 weeks (D) and 3 weeks (E) post-EDN1 treatment. Calcium transients per organoid was measured across 30 s. Each red dot represents one organoid sample analysed. n = 10 CCOs per group; mean ± s.e.m. Data information: Statistical analysis was performed using Students two-tailed T test. *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001