Fig. 4

Additional soluble factors secreted by MSCs contribute to suppression of HNT CD4⁺ T cell responses. Isolated mitochondria from MSC-A were transferred by mitoception to naïve HNT CD4⁺ T cells. 12 h later, mitocepted or mock mitocepted HNT CD4⁺ T cells were activated with anti-CD3 and anti-CD28 mAbs and cultured during 4 days in the presence of MSC conditioned media (Sup) or fresh MSC media. Data from 4 independent experiments is presented. A Absolute numbers of harvested HNT CD4⁺ T cells. Values are represented as mean ± SEM. B Expression of CD25 and CD40L in activated HNT CD4⁺ T cells. Mean fluoresce intensity (MFI) minus that of the isotype controls is indicated. Values are represented as mean ± SEM. C Activated HNT CD4⁺ T cells were restimulated with PMA and ionomycin in the presence of brefeldin A and production of intracellular IFNγ was assessed by FACS. Percentage of cytokine-producing T cells is indicated. Values are represented as mean ± SEM