Fig. 3

Proof of efficient β-cell induction by CDK8/19 inhibition. a Representative dot plots from the FCM analysis (left, 0.3 μM TAK-583; 3 nM BI-1347; and 0.1 μM senexin B) and dose–response relationships describing the induction of β-cells by CDK8/19 inhibitors (right). Data are shown as the mean ± SD of three independent experiments. *P < 0.05 and **P < 0.01 versus cells that were not treated by either ALK5iII or CDK8/19 inhibitors; Dunnett’s test. b Schematic docking model of a pair of on-target pharmacological tools, TR06096159 and TR05978156, bound to CDK8 (modified from the previous report [32]). c Proportions of β-cells differentiated with TR06096159, which binds CDK8, and TR05978156, which does not bind CDK8. Data are shown as the mean ± SD from three independent experiments. *P < 0.05 and **P < 0.01 versus untreated cells; Dunnett’s test