Fig. 3

ABCB5⁺ MSCs alleviated hypoxia-induced ROS burden in HUVECs. A, B HUVECs expressing roGFP3 were treated with 80 μM CoCl₂, or 120 μM DFO or 100 μM H₂O₂ in medium for 4 h. H₂O₂ was used as positive control. Thereafter, HUEVCs were either co-cultured with ABCB5⁺ MSCs (CoCl₂-CO; DFO-CO) or cultured in ABCB5⁺ MSC-CM (CoCl₂-CM; DFO-CM) for 24 h. The ratio of florescence intensity Ex 395 nm and at Ex 485 nm, and the emission at 520 nm was measured by a Tecan multimode reader every 2 h and ratio was calculated. A ROS changes in HUVECs treated by CoCl₂, CoCl₂-CM and CoCl₂-CO treatments; B DFO, DFO-CM and DFO-CO treatments. Data is represented as mean ± SD, n = 3 in each group