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Fig. 5 | Stem Cell Research & Therapy

Fig. 5

From: ABCB5+ mesenchymal stromal cells therapy protects from hypoxia by restoring Ca2+ homeostasis in vitro and in vivo

Fig. 5

ABCB5+ conditioned medium and co-culture both rescue metabolic activity and migration properties of hypoxic HUVECs. A–C HUVECs were treated either with 80 μM CoCl2 or 120 μM DFO for 4 h to induce hypoxia. HUVECs were also treated with 3 μM of TG. Afterward, HUVECs were either co-cultured with ABCB5+ MSCs (CoCl2-CO; DFO-CO; TG-CO) or cultured in ABCB5+ MSC conditioned medium (CoCl2-CM; DFO-CM; TG-CM) for 24 h. HUVECs without treatment served as control. Cellular metabolic activity of HUVECs was measured by MTT assay which is an indirect indicator of cell viability. HUVECs metabolic activity under A CoCl2, CoCl2-CM and CoCl2-CO treatments; B DFO, DFO-CM and DFO-CO treatments; C TG, TG-CM and TG-CO treatments. D Representative figure showing HUVECs migration under different treatments (scale bar is 200 μm). E–G Scratch assays were used to study migratory properties of HUVECs. After treatment with 80 μM CoCl2 or 120 μM DFO or 3 μM TG for 4 h, HUVECs monolayer was scratched along a straight line. Afterward, HUVECs were either co-cultured with the ABCB5+ cells (CoCl2-CO; DFO-CO; TG-CO) or cultured with ABCB5+ conditioned medium (CoCl2-CM; DFO-CM; TG-CM). The photos of the monolayer were taken at 0 h, 6 h, 12 h, and 24 h by an inverted microscope (Axiovert 200 M; Zeiss, Jena, Germany). The gap area was quantitatively evaluated using NIH ImageJ software (version 1.52, Bethesda, USA). Migration rate of HUVECs under E CoCl2, CoCl2-CM and CoCl2-CO treatments; F DFO, DFO-CM and DFO-CO treatments; G TG, TG-CM and TG-CO treatments. Data is represented as mean ± SD, n = 3 in each group, ns, not significant, *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001

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