Table 1 Characteristics of mice trials
References | Location | Animal sex | Animal species | Animal year | Group | Type and source of NSPC | Way of administrated | Delivered location | Dose of NSPC | Immunosuppression | Assessment time | Parameter |
|---|---|---|---|---|---|---|---|---|---|---|---|---|
Huang [15] | China | Female | APPswe/PS1dE9 mice | 9 months | AD + PBS AD + NSC AD + nanoformulation-NSC AD + NEP–NSC | Mice NSC from hippocampus | Stereotactically transplanted | Hippocampus | 1 × 105 | N | 1 month 6 months | ①② |
Wu [16] | China | NA | Tg2576 mice | 16 months | Tg + Vehicle Tg + NSC Tg + BDNF-NSC | Mice NSC from postnatal day 1 hippocampus | Stereotactically transplanted | Hippocampus | 1 × 105 | N | 8 weeks | ①②④⑥ |
Lee [17] | Korea | male | ICR mice | NA | IBO acid +  NSC (n = 9) IBO acid +  PBS (n = 7) IBO acid + NGF-NSC (n = 9) | Human NSC from 14-week fetal brain | Stereotactically transplanted | Cortex | 2 × 105 | N | 4 weeks | ①|
McGinley [18] | USA | Male | APPswe/PS1dE9 mice | 12 weeks | AD + vehicle (n = 10) AD + NSC (n = 10) | Human cortex-derived NSC | Stereotactically transplanted | Fimbria fornix | 1.8 × 105 | Y | 16 weeks | ①②④⑤ |
Zhao [19] | China | male | SAMP8 mice | 8 months | SAMP8 + sham operation (n = 10) SAMP8 + NSC (n = 10) | Mice NSC from embryo | Stereotactically transplanted | Hippocampus | 5 × 105 | N | 15 days | ①⑥ |
Zhang [20] | China | male | APPswe/PS1dE9 mice | 12 months | Tg + PBS (n = 10) Tg + NSC (n = 10) | Mice NSC | Stereotactically transplanted | Hippocampus | 5 × 105 to 1 × 106 | N | 10 weeks | ①④ |
Zhang [21] | China | NA | APPswe/PS1dE9mice | 12 months | Tg + vehicle (n = 20) Tg + NSC (n = 20) | Mice NSC from 14 days embryo | Stereotactically transplanted | Hippocampus | 2.5–5 × 106 | N | 5 weeks 10 weeks | ①|
Mathew [22] | USA | NA | APP/PS1/tau 3 × Tg AD mice | 18 months | 3xTg-AD + vehicle (n = 9) 3xTg-AD + NSC (n = 18) | Mice NSC (postnatal day 1) | Stereotactically transplanted | Hippocampus | 1 × 105 | N | 1 month | ①|
Chen [23] | China | Male | APP/PS1/tau 3 × Tg AD mice | 12 months | 3xTg-AD + PBS (n = 10) 3xTg-AD + NSC (n = 10) | Mice NSC from hippocampus and subependymal zone of 12.5 days fetal brain | Stereotactically transplanted | Hippocampus | 2 × 106 | N | 8 weeks | ①|
Lu [24] | China | male | APPswe/PS1dE9 mice | 3.5 months | AD + saline AD + NSC | Human NSC from hippocampus of 6–8-week embryos | Intranasally transplanted | Nasal cavity | 1 × 106, 4 times | Y | 3 months 4 months | ①②④⑤⑥ |
Zhang [25] | China | NA | Tg-tau mice | 40 weeks | Tg + PBS (n = 11) Tg + NSC (n = 11) | Mice NSC from hippocampus on postnatal day1 | Stereotactically transplanted | Hippocampus | 2 × 105 | N | 4 weeks | ①③ |
Zhang [26] | China | male | APPswe/PS1dE9 mice | 12 months | AD + PBS (n = 20) AD + NSC (n = 20) | Mice NSC from embryonic day 14 | Stereotactically transplanted | Hippocampus | 5 × 105to 1 × 106 | N | 10 weeks | ①②⑤ |
Zhang [27] | China | NA | APPswe/PS1dE9 mice | 10 months | AD + Vehicle (n = 15) AD + NSC (n = 15) | Mice NSC from embryonic day 14 | Stereotactically transplanted | Hippocampus | 1 × 106 | N | 8 weeks | ①④ |
Zhang [28] | China | NA | APPswe/PS1dE9 mice | 12 months | AD + Vehicle (n = 20) AD + NSC (n = 20) | Mice NSC from embryonic day 14 | Stereotactically transplanted | Hippocampus | 5 × 105 to 1 × 106 | N | 8 weeks | ①②④⑥ |
Zhou [29] | China | male | SAMP8 mice | 8 months | SAMP8 control (n = 10) SAMP8 + NSC (n = 10) SAMP8 SAMP8 + NSC + HuangDiSan (n = 10) | Mice NSC from embryonic day 12–16 | Stereotactically transplanted | Hippocampus | 1 × 106 | N | 15 days | ①④ |
Ofra [30] | Israel | male | APPswe/PS1dE9 mice | 11 months | AD + sham AD + NPC AD + IL-1ra-NPC | Mice NPC | Stereotactically transplanted | Hippocampus | 4000 spheres | N | 1 month | ①②⑥ |
Armijo [31] | USA | male and female | APP/PS1/tau 3 × Tg AD mice | 17 months | 3xTg-AD + PBS 3xTg-AD + NPC | Mice NPC from tail-tip fibroblasts | Stereotactically transplanted | Hippocampus | 5 × 105 | N | 1 month 2 months | ②③ |
Lee [32] | Korea | NA | NSE/APPsw transgenic mice | 13 months | APP + Vehicle APP + NSC | Human NSC from 13-week fetal brain | ICV | Lateral ventricles | 5 × 105 | Y | 6 weeks 12 weeks | ①②③④⑤⑥ |
Li [33] | China | male and female | APPswe/PS1dE9 mice | 12 months | AD + Vehicle (n = 10) AD + NSC (n = 10) | Mice NSC from embryonic day 14 | Stereotactically transplanted | Hippocampus | 2.5–5 × 106 | N | 3 weeks | ①⑤⑥ |
Li [34] | China | NA | APPswe/PS1dE9 mice | NA | AD + Saline AD + NSC AD + CSeM/let-7b NPs-NSC | Mice NSC from hippocampus | Stereotactically transplanted | NA | NA | N | 30 days | ①⑥ |
Lilja [35] | Sweden | male and female | Tg2576 Mice | 6–9 months | Tg + Vehicle + saline (n = 9) Tg + NSC + saline (n = 9) Tg + NSC + JN403 (n = 5) Tg + NSC + ( +)-phenserine (n = 7) | Human NSC | Stereotactically transplanted | Hippocampus | 2.5 × 104 | N | 5 weeks | ①② |
Park [36] | Korea | NA | APPswe/PS1dE9 mice | 18 months | AD + saline (n = 10) AD + NSC (n = 10) AD + CHAT-NSC (n = 10) | Human NSC from 15-week fetal brain | ICV | Lateral ventricles | 1 × 106 | N | 4 weeks | ①②⑥ |