Fig. 5

CCR2 antagonist restrained neuroblasts migration in TBI mice (day 7). Coronal sections of forebrain were immunostained with anti-DCX antibody (red, neuroblasts) and DAPI (blue, nucleus). White arrows: DCX⁺ cells. A1, a1, a1-1 Photomicrograph showing the distribution of DCX⁺ cells in the TBI group. The DCX positive cells were found in SVZ, CC and injury cortex in the TBI-CCR2 antagonist group. Scale bar = 12.5 μm (A1), 25 μm (a1), 50 μm (a1-1). A2, a2 Photomicrograph showing the distribution of DCX⁺ cells in the TBI-CCR2 antagonist group. The DCX positive cells were found only in SVZ and CC in the TBI group. Scale bar = 12.5 μm (A2), 25 μm (a2). B The number of migrating cells (DCX⁺ cells) in the CC and cortex was significantly smaller in the TBI-CCR2 antagonist group than in the TBI group (P < 0.05). Data are expressed as the mean ± SEM, n = 6 for each time-point. *, P < 0.05; Student’s t-test. CCI: controlled cortical impact; TBI: traumatic brain injury; CC chemokine ligand 2 = CCL2; DAPI: 4,6-Diamidino-2-phenylindole; CC: corpus callosum